护理专业大学生的复原力状况

目的:研究护理专业大学生复原力的情况,分析影响其复原力的因素,进而有针对性地对大学生进行必要的心理干预及预防,为有效开展大学生心理健康教育提供参考和依据。方法:在广东药学院大一~大四护理专业本科生中,随机抽取358名进行问卷调查,采用由刘兰兰编制的大学生复原力量表,并用SPSS 19.0做统计分析。结果:1护理本科生在性别方面总体复原力有显著差异,女生的总体复原力水平高于男生(F=5.001,P0.05);2在社会交往方面,不同年级存在显著差异,四年级的复原力水平最高(F=7.687,P0.05);在家庭支持方面,不同性别间的差异有统计学意义(t=2.251,P0.05);独生子女与非独生子女间的差异有统计学意义(t=2.203,P0.05)。在朋友支持方面,不同性别间的差异有统计学意义(t=3.629,P0.05);独生子女与非独生子女间的差异有统计学意义(t=1.990,P0.05)。在自我掌控方面,不同年级间的差异有统计学意义(F=3.045,P0.05);不同性别间的差异有统计学意义(t=2.105,P0.05)。结论:护理专业女生比男生的总体复原力水平高。社会交往、家庭支持、朋友支持、自我掌控等维度影响大学生复原力水平。     

Comparison of Fluorescence Microscopy and Different Growth Media Culture Methods for Acanthamoeba Keratitis Diagnosis

Acanthamoeba keratitis (AK), a potentially blinding infection of the cornea, is caused by a free-living protozoan. Culture and microscopic examination of corneal scraping tissue material is the conventional method for identifying Acanthamoeba. In this article, we compared several methods for AK diagnosis of 32 patients: microscopic examination using fluorescent dye, specific culture on growth media—non-nutrient agar (NNA), culture on liquid growth media—peptone yeast glucose (PYG), and TYI-S-33. AK was found in 14 patients. Thirteen of the specimens were found AK positive by fluorescence microscopic examination, 11 specimens were found AK positive on PYG growth media, and 9 specimens were found AK positive on TYI-S-33 growth media. Only five specimens were found AK positive on NNA growth media. Therefore, we recommend using fluorescence microscopy technique and culture method, especially PYG liquid media.     

Effects of 60-day bed rest with and without exercise on cellular and humoral immunological parameters

Exercise at regular intervals is assumed to have a positive effect on immune functions. Conversely, after spaceflight and under simulated weightlessness (e.g., bed rest), immune functions can be suppressed. We aimed to assess the effects of simulated weightlessness (Second Berlin BedRest Study; BBR2-2) on immunological parameters and to investigate the effect of exercise (resistive exercise with and without vibration) on these changes. Twenty-four physically and mentally healthy male volunteers (20–45 years) performed resistive vibration exercise (n=7), resistance exercise without vibration (n=8) or no exercise (n=9) within 60 days of bed rest. Blood samples were taken 2 days before bed rest, on days 19 and 60 of bed rest. Composition of immune cells was analyzed by flow cytometry. Cytokines and neuroendocrine parameters were analyzed by Luminex technology and ELISA/RIA in plasma. General changes over time were identified by paired t-test, and exercise-dependent effects by pairwise repeated measurements (analysis of variance (ANOVA)). With all subjects pooled, the number of granulocytes, natural killer T cells, hematopoietic stem cells and CD45RA and CD25 co-expressing T cells increased and the number of monocytes decreased significantly during the study; the concentration of eotaxin decreased significantly. Different impacts of exercise were seen for lymphocytes, B cells, especially the IgD⁺ subpopulation of B cells and the concentrations of IP-10, RANTES and DHEA-S. We conclude that prolonged bed rest significantly impacts immune cell populations and cytokine concentrations. Exercise was able to specifically influence different immunological parameters. In summary, our data fit the hypothesis of immunoprotection by exercise and may point toward even superior effects by resistive vibration exercise.